Title |
The quorum sensing regulator OpaR is a repressor of polar flagellum genes in Vibrio parahaemolyticus |
Author |
Renfei Lu1, Junfang Sun1, Yue Qiu3, Miaomiao Zhang3, Xingfan Xue3, Xue Li1, Wenhui Yang4, Dongsheng Zhou4, Lingfei Hu4*, and Yiquan Zhang2,3* |
Address |
1Department of Clinical Laboratory, Nantong Third Hospital Affiliated to Nantong University, Nantong 212006, P. R. China, 2Wuxi School of Medicine, Jiangnan University, Wuxi 214122, P. R. China, 3School of Medicine, Jiangsu University, Zhenjiang 212013, P. R. China, 4State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, P. R. China |
Bibliography |
Journal of Microbiology, 59(7),651–657, 2021,
|
DOI |
10.1007/s12275-021-0629-3
|
Key Words |
Vibrio parahaemolyticus, polar flagellum, quorum
sensing, OpaR |
Abstract |
Vibrio parahaemolyticus possesses two types of flagella: a
single polar flagellum (Pof) for swimming and the peritrichous
lateral flagella (Laf) for swarming. Expression of Laf
genes has previously been reported to be regulated by the quorum
sensing (QS) regulators AphA and OpaR. In the present
study, we showed that OpaR, the QS regulator at high cell density
(HCD), acted as a negative regulator of swimming motility
and the transcription of Pof genes in V. parahaemolyticus.
OpaR bound to the promoter-proximal DNA regions
of flgAMN, flgMN, and flgBCDEFGHIJ within the Pof gene
loci to repress their transcription, whereas it negatively regulates
the transcription of flgKL-flaC in an indirect manner.
Thus, this work investigated how QS regulated the swimming
motility via direct action of its master regulator OpaR on
the transcription of Pof genes in V. parahaemolyticus. |