Title |
Brevibacterium limosum sp. nov., Brevibacterium pigmenatum sp. nov., and Brevibacterium atlanticum sp. nov., three novel dye decolorizing actinobacteria isolated from ocean sediments |
Author |
Shengxiang Pei1,2, Siwen Niu1, Fuquan Xie1, Wenjing Wang1, Shuang Zhang1, and Gaiyun Zhang1* |
Address |
1Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian, P. R. China, 2State Key Laboratory of Marine Environmental Science, College of Ocean and EarthSciences, Xiamen University, Xiamen 361102, Fujian, P. R. China |
Bibliography |
Journal of Microbiology, 59(10),898-910, 2021,
|
DOI |
10.1007/s12275-021-1235-0
|
Key Words |
Brevibacterium limosum sp. nov., Brevibacterium
pigmenatum sp. nov., Brevibacterium atlanticum sp. nov.,
genomic analysis, dye decolorization |
Abstract |
During a study of the marine actinobacterial biodiversity, a
large number of Brevibacterium strains were isolated. Of these,
five that have relatively low 16S rRNA gene similarity (98.5–
99.3%) with validly published Brevibacterium species, were
chosen to determine taxonomic positions. On the basis of 16S
rRNA gene sequence analysis and BOX-PCR fingerprinting,
strains o2T, YB235T, and WO024T were selected as representative
strains. Genomic analyses, including average nucleotide
identity (ANI) and digital DNA-DNA hybridization (dDDH),
clearly differentiated the three strains from each other and
from their closest relatives, with values ranging from 82.8%
to 91.5% for ANI and from 26.7% to 46.5% for dDDH that
below the threshold for species delineation. Strains YB235T,
WO024T, and o2T all exhibited strong and efficient decolorization
activity in congo red (CR) dyes, moderate decolorization
activity in toluidine blue (TB) dyes and poor decolorization
in reactive blue (RB) dyes. Genes coding for peroxidases
and laccases were identified and accounted for these strains’
ability to effectively oxidize a variety of dyes with different
chemical structures. Mining of the whole genome for secondary
metabolite biosynthesis gene clusters revealed the presence
of gene clusters encoding for bacteriocin, ectoine, NRPS,
siderophore, T3PKS, terpene, and thiopeptide. Based on the
phylogenetic, genotypic and phenotypic data, strains o2T,
YB235T and WO024T clearly represent three novel taxa within
the genus Brevibacterium, for which the names Brevibacterium
limosum sp. nov. (type strain o2T = JCM 33844T = MCCC
1A09961T), Brevibacterium pigmenatum sp. nov. (type strain
YB235T = JCM 33843T = MCCC 1A09842T) and Brevibacterium
atlanticum sp. nov. (type strain WO024T = JCM 33846T
= MCCC 1A16743T) are proposed. |