Title |
Characterization and bioefficacy of green nanosilver particles derived from fungicide-tolerant Tricho-fusant for efficient biocontrol of stem rot (Sclerotium rolfsii Sacc.) in groundnut (Arachis hypogaea L.) |
Author |
Darshna G. Hirpara, Harsukh P. Gajera*, Disha D. Savaliya, and Rushita V. Bhadani |
Address |
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh 362 001, Gujarat, India |
Bibliography |
Journal of Microbiology, 59(11),1031-1043, 2021,
|
DOI |
10.1007/s12275-021-1344-9
|
Key Words |
groundnut, stem rot, Tricho-Fu21, green nanosilver,
field efficacy, fungicides alternate |
Abstract |
An efficient and eco-friendly bioefficacy of potent Trichofusant
(Fu21) and its green nanosilver formulation against
stem rot (Sclerotium rolfsii) in groundnut was established.
Fu21 demonstrated higher in-vitro growth inhibition of pathogen
with better fungicide tolerance than the parental strains.
The green nanosilver particles were synthesized from the extracellular
metabolites of Fu21 and characterized for shape
(spherical, 59.34 nm in scanning electron microscope), purity
(3.00 KeV, energy dispersive X-ray analysis), size (54.3 nm
in particle size analyzer), and stability (53.7 mv, zeta). The field
efficacy study exhibited that the seedling emergence was high
in seeds treated with green nanosilver (minimum inhibitory
concentration-[MIC] 20 μg Ag/ml), and a low disease severity
index of stem rot during the crop growth was followed by the
live antagonist (Fu21) in addition to seed treatment with a
fungicide mix under pathogen infestation. The seed quality
analysis of harvested pods revealed a high oil content with
balanced fatty acid composition (3.10 oleic/linoleic acid ratio)
in green nanosilver treatment under pathogen infestation.
The residual analysis suggested that green nanosilver applied
at the MIC level as seed treatment yielded similar effects as the
control for silver residue in the harvested groundnut seeds.
The green nanosilver at MIC has a high pod-yield under S.
rolfsii infestation, demonstrating green chemistry and sustainability
of the nanoproduct. |