Title |
Genome information of the cellulolytic soil actinobacterium Isoptericola dokdonensis DS-3 and comparative genomic analysis of the genus Isoptericola |
Author |
Yurim Bae1, Sujin Lee1, Kitae Kim2, Hyun-Kwon Lee2, Soon-Kyeong Kwon1, and Jihyun F. Kim2,3* |
Address |
1Division of Applied Life Science (BK21), Gyeongsang National University, Jinju 52828, Republic of Korea, 2Department of Systems Biology, Division of Life Sciences, Yonsei University, Seoul 03722, Republic of Korea, 3Strategic Initiative for Microbiomes in Agriculture and Food, Yonsei University, Seoul 03722, Republic of Korea |
Bibliography |
Journal of Microbiology, 59(11),1010-1018, 2021,
|
DOI |
10.1007/s12275-021-1452-6
|
Key Words |
actinobacteria, comparative genomics, Isoptericola,
CAZyme, PacBio sequencing |
Abstract |
The actinobacterial group is regarded as a reservoir of biologically
active natural products and hydrolytic enzymes with
the potential for biomedical and industrial applications. Here,
we present the complete genome sequence of Isoptericola
dokdonensis DS-3 isolated from soil in Dokdo, small islets
in the East Sea of Korea. This actinomycete harbors a large
number of genes encoding carbohydrate-degrading enzymes,
and its activity to degrade carboxymethyl cellulose into glucose
was experimentally evaluated. Since the genus Isoptericola was
proposed after reclassification based on phylogenetic analysis,
strains of Isoptericola have been continuously isolated from
diverse environments and the importance of this genus in the
ecosystem has been suggested by recent culturomic or metagenomic
studies. The phylogenic relationships of the genus
tended to be closer among strains that had been isolated from
similar habitats. By analyzing the properties of published genome
sequences of seven defined species in the genus, a large
number of genes for carbohydrate hydrolysis and utilization,
as well as several biosynthetic gene clusters for secondary
metabolites, were identified. Genomic information of I. dokdonensis
DS-3 together with comparative analysis of the genomes
of Isoptericola provides insights into understanding
this actinobacterial group with a potential for industrial applications. |