Title |
Short-chain fatty acids inhibit the biofilm formation of Streptococcus gordonii through negative regulation of competence-stimulating peptide signaling pathway |
Author |
Taehwan Park1, Jintaek Im1, A Reum Kim1, Dongwook Lee1, Sungho Jeong1, Cheol-Heui Yun2,3, and Seung Hyun Han1* |
Address |
1Department of Oral Microbiology and Immunology, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea, 2Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea, 3Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea |
Bibliography |
Journal of Microbiology, 59(12),1142–1149, 2021,
|
DOI |
10.1007/s12275-021-1576-8
|
Key Words |
Streptococcus gordonii, biofilm, short-chain fatty
acids, quorum-sensing, competence-stimulating peptide |
Abstract |
Streptococcus gordonii, a Gram-positive commensal bacterium,
is an opportunistic pathogen closely related to initiation
and progression of various oral diseases, such as periodontitis
and dental caries. Its biofilm formation is linked
with the development of such diseases by enhanced resistance
against antimicrobial treatment or host immunity. In the
present study, we investigated the effect of short-chain fatty
acids (SCFAs) on the biofilm formation of S. gordonii. SCFAs,
including sodium acetate (NaA), sodium propionate (NaP),
and sodium butyrate (NaB), showed an effective inhibitory
activity on the biofilm formation of S. gordonii without reduction
in bacterial growth. SCFAs suppressed S. gordonii
biofilm formation at early time points whereas SCFAs did
not affect its preformed biofilm. A quorum-sensing system
mediated by competence-stimulating peptide (CSP) is known
to regulate biofilm formation of streptococci. Interestingly,
SCFAs substantially decreased mRNA expression of comD
and comE, which are CSP-sensor and its response regulator
responsible for CSP pathway, respectively. Although S. gordonii
biofilm formation was enhanced by exogenous synthetic
CSP treatment, such effect was not observed in the
presence of SCFAs. Collectively, these results suggest that
SCFAs have an anti-biofilm activity on S. gordonii through
inhibiting comD and comE expression which results in negative
regulation of CSP quorum-sensing system. SCFAs could
be an effective anti-biofilm agent against S. gordonii for the
prevention of oral diseases. |