Title |
Direct current exerts electricidal and bioelectric effects on Porphyromonas gingivalis biofilms partially via promoting oxidative stress and antibiotic transport |
Author |
Peihui Zou1,2, Peng Li1,2, Jia Liu1,2, Pei Cao1,2, and Qingxian Luan1,2 |
Address |
1Department of Periodontology, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing 100081, P. R. China, 2Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Beijing 100081, P. R. China |
Bibliography |
Journal of Microbiology, 60(1),70-78, 2022,
|
DOI |
10.1007/s12275-022-1238-5
|
Key Words |
direct current, Porphyromonas gingivalis, biofilms,
electricidal effect, bioelectric effect |
Abstract |
Low electric current can inhibit certain microbial biofilms and
enhance the efficacy of antimicrobials against them. This study
investigated the electricidal and bioelectric effects of direct
current (DC) against Porphyromonas gingivalis biofilms as
well as the underlying mechanisms. Here, we firstly showed
that DC significantly suppressed biofilm formation of P. gingivalis
in time- and intensity-dependent manners, and markedly
inhibited preformed P. gingivalis biofilms. Moreover,
DC enhanced the killing efficacy of metronidazole (MTZ) and
amoxicillin with clavulanate potassium (AMC) against the
biofilms. Notably, DC-treated biofilms displayed upregulated
intracellular ROS and expression of ROS related genes (sod,
feoB, and oxyR) as well as porin gene. Interestingly, DC-induced
killing of biofilms was partially reversed by ROS scavenger
N-dimethylthiourea (DMTU), and the synergistic effect
of DC with MTZ/AMC was weakened by small interfering
RNA of porin gene (si-Porin). In conclusion, DC can
exert electricidal and bioelectric effects against P. gingivalis
biofilms partially via promotion of oxidative stress and antibiotic
transport, which offers a promising approach for effective
management of periodontitis. |