| Title | Improved tolerance of recombinant Chlamydomonas rainhardtii with putative 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase from Pyropia yezoensis to nitrogen starvation |
|---|---|
| Author | Seo-jeong Park1, Joon Woo Ahn2, and Jong-il Choi1* |
| Address | 1Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea, 2Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea |
| Bibliography | Journal of Microbiology, 60(1),63-69, 2022, |
| DOI | 10.1007/s12275-022-1491-7 |
| Key Words | 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase, nitrogen starvation, Chlamydomonas reinhardtii, lipid accumulation, Pyropia yezoensis |
| Abstract | In a previous study, a putative 2-amino-3-carboxymuconate- 6-semialdehyde decarboxylase (ACMSD) was highly expressed in a mutant strain of Pyropia yezoensis, which exhibited an improved growth rate compared to its wild strain. To investigate the functional role of the putative ACMSD (Pyacmsd) of P. yezoensis, the putative Pyacmsd was cloned and expressed in Chlamydomonas reinhardtii. Recombinant C. reinhardtii cells with Pyacmsd (Cr_Pyacmsd) exhibited enhanced tolerance compared to control C. reinhardtii cells (Cr_control) under nitrogen starvation. Notably, Cr_Pyacmsd cells showed accumulation of lipids in nitrogen-enriched conditions. These results demonstrate the role of Pyacmsd in the generation of acetyl-coenzyme A. Thus, it can be used to enhance the production of biofuel using microalgae such as C. reinhardtii and increase the tolerance of other biological systems to nitrogendeficient conditions. |