Title |
Devosia rhizoryzae sp. nov., and Devosia oryziradicis sp. nov., novel plant growth promoting members of the genus Devosia, isolated from the rhizosphere of rice plants |
Author |
Geeta Chhetri, Inhyup Kim, Minchung Kang, Jiyoun Kim, Yoonseop So, and Taegun Seo* |
Address |
Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea |
Bibliography |
Journal of Microbiology, 60(1),1-10, 2022,
|
DOI |
10.1007/s12275-022-1474-8
|
Key Words |
Devosia, indole acetic acid, siderophore, L-tryptophan,
paddy field, PGPR, carotenoid, flexirubin |
Abstract |
Two novel Gram-negative, aerobic, asporogenous, motile, rodshaped,
orange and white pigmented, designated as LEGU1T
and G19T, were isolated from the roots of rice plants, collected
from Goyang, South Korea. Phylogenetic analysis based on
their 16S rRNA gene sequences revealed that they belonged to
the genus Devosia and formed a different lineage and clusters
with different members of the genus Devosia. These strains
shared common chemotaxonomic features. In particular, they
had Q-10 as the sole quinone, phosphatidylglycerol, diphosphatidylglycerol
as the principal polar lipids and C16:0, C18:1
ω7c 11-methyl and summed feature 8 (comprising C18:1 ω7c/
C18:1 ω6c) as the main fatty acids. The draft genome sequences
of strains LEGU1T and G19T were 3,524,978 and 3,495,520 bp
in size, respectively. Their average nucleotide identity (ANI)
and digital DNA-DNA hybridization (dDDH) values were
72.8–81.9% and 18.7–25.1%, respectively, with each other and
type strains of related species belonging to the genus Devosia,
suggesting that these two strains represent novel species. The
G + C content of strains LEGU1T and G19T were 62.1 and
63.8%, respectively. Of the two strains, only LEGU1T produced
carotenoid and flexirubin-type pigment. Both strains
produced siderophore and indole acetic acid (IAA) in the
presence of L-tryptophan. Siderophore biosynthesis genes,
auxin responsive genes and tryptophan biosynthesis genes
were present in their genomes. The present study aimed to
determine the detailed taxonomic positions of the strains
using the modern polyphasic approach. Based on the results
of polyphasic analysis, these strains are suggested to be two
novel bacterial species within the genus Devosia. The proposed
names are D. rhizoryzae sp. nov., and Devosia oryziradicis
sp. nov., respectively. The plant growth promoting effects
of these strains suggest that they can be exploited to improve
rice crop productivity. The type strain of D. rhizoryzae
is LEGU1T (KCTC 82712T = NBRC 114485T) and D. oryziradicis
is G19T (KCTC 82688T = NBRC 114842T). |