Title Characterization of components of a reducing system for SoxR in the cytoplasmic membrane of Escherichia coli
Author Kang-Lok Lee1*, Kyung-Chang Lee2, Joon-Hee Lee3, and Jung-Hye Roe2
Address 1Department of Biology Education, IALS, Gyeongsang National University, Jinju 52828, Republic of Korea, 2Laboratory of Molecular Microbiology, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea, 3College of Pharmacy, Pusan National University, Pusan 46241, Republic of Korea
Bibliography Journal of Microbiology, 60(4),387-394, 2022,
DOI 10.1007/s12275-022-1667-1
Key Words Fe-S, NADH, RseC, ApbE, Rsx, Rnf
Abstract A reducing system of SoxR, a regulator of redox-active molecules, was identified as rsxABCDGE gene products and RseC in Escherichia coli through genetic studies. We found that ApbE was an additional component of the reducer system. Bacterial two hybrid analysis revealed that these proteins indeed had multiple interactions among themselves. RseC and RsxB formed the core of the complex, interacting with more than five other components. RsxC, the only cytoplasmic component of the system, interacted with SoxR. It might be linked with the rest of the complex via RsxB. Membrane fractions containing the wild type complex but not the mutant complex reduced purified SoxR using NADH as an electron source. These results suggest that Rsx genes, RseC, and ApbE can form a complex using NAD(P)H to reduce SoxR.