| Title | Flaviflexus equikiangi sp. nov. isolated from faeces of Equus kiang (Tibetan wild ass) and carrying a class 1 integron gene cassette in its genome |
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| Author | Caixin Yang1,2, Xingxing Lian1,2, Yanpeng Cheng1,2,3, Yifan Jiao1,2, Jing Yang2,4,5, Kui Dong1,2,6, Shan Lu2,4,5, Xin-He Lai7, Dong Jin2,4,5, Han Zheng2,4, Ji Pu2, Suping Wang1, Liyun Liu2,5, and Jianguo Xu1,2,4,5,8* |
| Address | 1Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, P. R. China, 2State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P. R. China, 3Shenzhen Center for Disease Control and Prevention, Shenzhen 518000, PR China, 4Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, P. R. China, 5Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 102206, P. R. China, 6Shanxi Eye Hospital, Taiyuan, Shanxi 030001, P. R. China, 7Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, P. R. China |
| Bibliography | Journal of Microbiology, 60(6),585-593, 2022, |
| DOI | 10.1007/s12275-022-1673-3 |
| Key Words | Flaviflexus, Equus kiang, novel species, class 1 integron |
| Abstract | Two Gram-stain-positive, catalase-negative, non-spore-forming, cocci-shaped strains (dk850T and JY899) were isolated from the feces of Equus kiang in the Qinghai-Tibet Plateau of China. 16S rRNA gene sequence-based phylogenetic analyses showed that strains dk850T and JY899 belong to the genus Flaviflexus, closest to F. salsibiostraticola KCTC 33148T, F. ciconiae KCTC 49253T and F. huanghaiensis H5T. The DNA G + C content of strain dk850T was 62.9%. The digital DNADNA hybridization values of strain dk850T with the closely related species were below the 70% threshold for species demarcation. The two strains grew best at 28°C on brain heart infusion (BHI) agar with 5% sheep blood. All strains had C18:1ω9c and C16:0 as the major cellular fatty acids. MK-9(H4) was the major menaquinone in strain dk850T. The major polar lipids included diphosphatidylglycerol and an unidentified phospholipid. Strains dk850T and JY899 were identified as carrying a class 1 integron containing the aminoglycoside resistance gene aadA11, both strains were resistant to spectinomycin and streptomycin. Based on several lines of evidence from phenotypic and phylogenetic analyses, strains dk850T and JY899 represent a novel species of the genus Flaviflexus, for which the name Flaviflexus equikiangi sp. nov. is proposed. The type strain is dk850T (= CGMCC 1.16593T = JCM 33598T). |