| Title | Isolation of a novel Lactiplantibacillus plantarum strain resistant to nitrite stress and its transcriptome analysis |
|---|---|
| Author | Chae Young Kwon1,2, Kyoung Jin Choi1,2, Dongeun Yong3,4, Ji-Eun Kim1,5,6*, and Sang Sun Yoon1,2,5,6* |
| Address | 1Department of Microbiology and Immunology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, 2Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, 3Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, 4Research Institute of Bacterial resistance, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, 5Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, 6Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea |
| Bibliography | Journal of Microbiology, 60(7),715-726, 2022, |
| DOI | 10.1007/s12275-022-2221-x |
| Key Words | Lactiplantibacillus plantarum, RNS, human isolate, transcriptomic study |
| Abstract | Nitric oxide (NO) is a reactive nitrogen species (RNS) that plays a vital role in regulating inflammatory processes. Under abnormal conditions, excessive NO levels can promote the oxidation of cellular components, which may cause or exacerbate diseases such as hypertension, cardiovascular dysfunction, and inflammatory bowel disease (IBD). Previous studies have shown that reducing NO levels in the lumen can attenuate the clinical symptoms of IBD. Thus, we aimed to identify bacteria that can reduce RNS and that can be used as valuable probiotics. In this study, we isolated bacteria resistant to nitrite stress from human feces and used 16S and whole-genome sequencing to identify them as Lactiplantibacillus plantarum LP7 (LP7). The ability to survive at high nitrite levels and to decrease them was greater in the LP7 strain than in the reference strain L. plantarum ATCC14917 (ATCC- 14917). To characterize the LP7 genome in more detail, we performed a comparative genome analysis. However, the unique genes that directly confer the ability to withstand nitrite stress were not present in the LP7 genome. Furthermore, we performed transcriptomic analysis of LP7 and ATCC14917 cells treated with nitrite. We found that the expression levels of genes involved in the cell division process were induced in LP7, which showed a more regular rod-shape than ATCC- 14917. This could explain why LP7 can survive better than ATCC14917 under nitrite stress. Based on its ability to survive better in nitrite stress and decrease nitrite concentration, we suggest that LP7 could be a valuable probiotic strain. |