Title |
Brachybacterium kimchii sp. nov. and Brachybacterium halotolerans subsp. kimchii subsp. nov., isolated from the Korean fermented vegetables, kimchi, and description of Brachybacterium halotolerans subsp. halotolerans subsp. nov. |
Author |
Yujin Kim1, Yeon Bee Kim1, Juseok Kim1,2, Joon Yong Kim1,2, Tae Woong Whon1, Won-Hyong Chung3, Eun-Ji Song3, Young-Do Nam3, Se Hee Lee1*, and Seong Woon Roh1,2* |
Address |
1Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea, 2Microbiome Research Team, LISCure Biosciences Inc., Seongnam 13486, Republic of Korea, 3Research Group of Healthcare, Korea Food Research Institute, Wanju 55365, Republic of Korea |
Bibliography |
Journal of Microbiology, 60(7),678-688, 2022,
|
DOI |
10.1007/s12275-022-1581-6
|
Key Words |
Brachybacterium kimchii, Brachybacterium halotolerans
subsp. kimchii, Brachybacterium halotolerans subsp.
halotolerans, CBA3104T, CBA3105T, kimchi, polyphasic taxonomy |
Abstract |
Two Gram-stain-positive, oxidase-negative, catalase-positive,
and coccus-shaped bacterial strains, designated CBA3104T
and CBA3105T, were isolated from kimchi. Strain CBA3104T
and CBA3105T grew at 10–35°C (optimum, 25°C and 30°C,
respectively), at pH 6.0–8.5 (optimum, pH 6.5), and in the
presence of 0–15% (w/v) NaCl (optimum, 5%). A phylogenetic
analysis based on 16S rRNA gene sequences revealed
that strain CBA3104T formed a distinct phylogenetic lineage
within the genus Brachybacterium whereas strain CBA3105T
was closely positioned with Brachybacterium halotolerans
MASK1Z-5T. The 16S rRNA gene sequence similarity between
strains CBA3104T and CBA3105T was 99.9%, but ANI
and dDDH values between strains CBA3104T and CBA3105T
were 93.61% and 51.5%, respectively. Strain CBA3104T showed
lower ANI and dDDH values than species delineation against
three closely related strains and type species of the genus
Brachybacterium, however, strain CBA3105T showed 96.63%
ANI value and 69.6% dDDH value with Brachybacterium
halotolerans MASK1Z-5T. Among biochemical analysis results,
strain CBA3104T could uniquely utilize bromo-succinic
acid whereas only strain CBA3105T was positive for alkaline
phosphatase and α-fucosidase among two novel strains, closely
related strains, and type species of the genus Brachybacterium.
Compared with strain CBA3105T and Brachybacterium
halotolerans JCM 34339T, strain CBA3105T was differentially
positive for acid production of D-arabinose, D-adonitol, and
potassium 5-ketogluconate and enzyme activity of β-glucuronidase.
Both strains contained menaquinone-7 as the dominant
quinone. The cell-wall peptidoglycan of two novel
strains contained meso-diaminopimelic acid. The major fatty
acids of strains CBA3104T and CBA3105T were anteiso-C15:0,
anteiso-C17:0, and iso-C16:0. The major polar lipids of both
strains were phosphatidylglycerol and diphosphatidylglycerol.
Strain CBA3104T possessed a uniquely higher abundance of
tRNA (97 tRNAs) than four Brachybacterium strains used for
comparative taxonomic analysis (54–62 tRNAs). Both the
CBA3104T and CBA3105T strain harbored various oxidoreductase,
transferase, hydrolase, and lyase as strain-specific
functional genes compared to closely related strains and Brachybacterium
type species. The results of biochemical/physiological,
chemotaxonomic, and genomic analyses demonstrated
that strains CBA3104T and CBA3105T represent a novel species
of the genus Brachybacterium and a novel subspecies of
B. halotolerans, respectively, for which the names Brachybacterium
kimchii sp. nov. and B. halotolerans subsp. kimchii
subsp. nov. are proposed. The type strains of the novel
species and the novel subspecies are CBA3104T (= KCCM
43417T = JCM 34759T) and CBA3105T (= KCCM 43418T =
JCM 34760T), respectively. |