Title |
Complete gammaproteobacterial endosymbiont genome assembly from a seep tubeworm Lamellibrachia satsuma |
Author |
Ajit Kumar Patra1, Yong min Kwon2, and Youngik Yang3* |
Address |
1Division of Ecoscience, Ewha Womans University, Seoul 03760, Republic of Korea, 2Department of Macrobial Resources, National Marine Biodiversity Institute of Korea, Seochun 33662, Republic of Korea, 3Department of Genetic Resources, National Marine Biodiversity Institute of Korea, Seochun 33662, Republic of Korea |
Bibliography |
Journal of Microbiology, 60(9),916-927, 2022,
|
DOI |
10.1007/s12275-022-2057-4
|
Key Words |
gammaproteobacterial symbiont, chemosynthetic
bacteria, seep tubeworm Lamellibrachia satsuma, host-microbe
interaction, energy metabolism, Oxford Nanopore Technologies,
complete genome, comparative genomics |
Abstract |
Siboglinid tubeworms thrive in hydrothermal vent and seep
habitats via a symbiotic relationship with chemosynthetic bacteria.
Difficulties in culturing tubeworms and their symbionts
in a laboratory setting have hindered the study of host-microbe
interactions. Therefore, released symbiont genomes are
fragmented, thereby limiting the data available on the genome
that affect subsequent analyses. Here, we present a complete
genome of gammaproteobacterial endosymbiont from the
tubeworm Lamellibrachia satsuma collected from a seep in
Kagoshima Bay, assembled using a hybrid approach that combines
sequences generated from the Illumina and Oxford Nanopore
platforms. The genome consists of a single circular chromosome
with an assembly size of 4,323,754 bp and a GC content
of 53.9% with 3,624 protein-coding genes. The genome
is of high quality and contains no assembly gaps, while the
completeness and contamination are 99.33% and 2.73%,
respectively. Comparative genome analysis revealed a total
of 1,724 gene clusters shared in the vent and seep tubeworm
symbionts, while 294 genes were found exclusively in L. satsuma
symbionts such as transposons, genes for defense mechanisms,
and inorganic ion transportations. The addition of
this complete endosymbiont genome assembly would be valuable
for comparative studies particularly with tubeworm symbiont
genomes as well as with other chemosynthetic microbial
communities. |