| Title | Sala cibi gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt |
|---|---|
| Author | Hye Seon Song1, Juseok Kim2, Yeon Bee Kim1, Se Hee Lee1, Tae Woong Whon1*, and Seong Woon Roh2* |
| Address | 1Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea, 2Microbiome Research Team, LISCure Biosciences Inc., Seongnam 13486, Republic of Korea |
| Bibliography | Journal of Microbiology, 60(9),899-904, 2022, |
| DOI | 10.1007/s12275-022-2137-5 |
| Key Words | halophilic archaea, Sala cibi, solar salt, polyphasic taxonomy |
| Abstract | Two novel halophilic archaeal strains, CBA1133T and CBA- 1134, were isolated from solar salt in South Korea. The 16S rRNA gene sequences of the isolates were identical to each other and were closely related to the genera Natronomonas (92.3–93.5%), Salinirubellus (92.2%), Halomarina (91.3– 92.0%), and Haloglomus (91.4%). The isolated strains were coccoid, Gram-stain-negative, aerobic, oxidase-positive, and catalase-negative. Growth occurred under temperatures of 25–50°C (optimum, 45°C), NaCl levels of 10–30% (optimum, 15%), pH levels of 6.0–8.5 (optimum, 7.0), and MgCl2 concentrations of 0–500 mM (optimum, 100 mM). Digital DNADNA hybridization values between the strains and related genera ranged from 18.3% to 22.7%. The major polar lipids of the strains were phosphatidyl glycerol, phosphatidyl glycerol phosphate methyl ester, and phosphatidyl glycerol sulfate. Genomic, phenotypic, physiological, and biochemical analyses of the isolates revealed that they represent a novel genus and species in the family Halobacteriaceae. The type strain is CBA1133T (= KACC 22148T = JCM 34265T), for which the name Sala cibi gen. nov., sp. nov. is proposed. |