| Title | Genome Sequencing Highlights the Plant Cell Wall Degrading Capacity of Edible Mushroom Stropharia rugosoannulata |
|---|---|
| Author | Mengpei Guo1, Xiaolong Ma1*, Yan Zhou2*, Yinbing Bian2, Gaolei Liu1, Yingli Cai1, Tianji Huang3, Hongxia Dong1, Dingjun Cai1, Xueji Wan1, Zhihong Wang1, Yang Xiao2, and Heng Kang2 |
| Address | 1Institute of Vegetable, Wuhan Academy of Agricultural Sciences, Wuhan 430065, Hubei, People’s Republic of China, 2Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, Hubei, People’s Republic of China, 3Hubei Changjiu Fungi Co. Ltd., Suizhou 431525, Hubei, People’s Republic of China |
| Bibliography | Journal of Microbiology, 61(1),83-93, 2023, |
| DOI | 10.1007/s12275-022-00003-7 |
| Key Words | Stropharia rugosoannulata · Whole-genome sequencing · Plant cell wall degrading enzymes · Comparative genomic analysis |
| Abstract | The basidiomycetous edible mushroom Stropharia rugosoannulata has excellent nutrition, medicine, bioremediation, and biocontrol properties. S. rugosoannulata has been widely and easily cultivated using agricultural by-products showing strong lignocellulose degradation capacity. However, the unavailable high-quality genome information has hindered the research on gene function and molecular breeding of S. rugosoannulata. This study provided a high-quality genome assembly and annotation from S. rugosoannulata monokaryotic strain QGU27 based on combined Illumina-Nanopore data. The genome size was about 47.97 Mb and consisted of 20 scaffolds, with an N50 of 3.73 Mb and a GC content of 47.9%. The repetitive sequences accounted for 17.41% of the genome, mostly long terminal repeats (LTRs). A total of 15,726 coding gene sequences were putatively identified with the BUSCO score of 98.7%. There are 142 genes encoding plant cell wall degrading enzymes (PCWDEs) in the genome, and 52, 39, 30, 11, 8, and 2 genes related to lignin, cellulose, hemicellulose, pectin, chitin, and cutin degradation, respectively. Comparative genomic analysis revealed that S. rugosoannulata is superior in utilizing aldehyde-containing lignins and is possible to utilize algae during the cultivation. |