| Title | Microbial Interaction is Among the Key Factors for Isolation of Previous Uncultured Microbes |
|---|---|
| Author | Chang Yan1,2, Jeffrey S. Owen3, Eun‑Young Seo2, Dawoon Jung2*, and Shan He2,4* |
| Address | 1Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, People’s Republic of China, 2Ningbo Institute of Marine Medicine, Peking University, Ningbo 315832, People’s Republic of China, 3Department of Environmental Science, Hankuk University of Foreign Studies, Yongin 17035, Republic of Korea, 4Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, People’s Republic of Chin |
| Bibliography | Journal of Microbiology, 61(7),655-662, 2023, |
| DOI | 10.1007/s12275-023-00063-3 |
| Key Words | Uncultured microbes · Microbial uncultivability · Microbial cultivation · Growth factors |
| Abstract | Pure cultivation of microbes is still limited by the challenges of microbial uncultivability, with most microbial strains unable to be cultivated under standard laboratory conditions. The experience accumulated from advanced techniques such as in situ cultivation has identified that microbial interactions exist in natural habitats but are absent in laboratory cultures. These microbial interactions are likely one of the key factors in isolating previously uncultured microbes. The need for better knowledge of the mechanisms operating in microbial interactions has led to various experiments that have utilized microbial interactions in different approaches to microbial cultivation. These new attempts to understand microbial interactions not only present a new perspective on microbial uncultivability but also provide an opportunity to access uncultured phylogenetically novel microbes with their potential biotechnology applications. In this review, we focus on studies of the mechanisms of microbial interaction where the growth of other microbes is affected. Additionally, we review some successful applications of microbial interactions in cultivation methods, an approach that can play an important role in the bioprospecting of untapped microbial resources. |