| Title | Genetic Characteristics and Phylogeographic Dynamics of Echovirus |
|---|---|
| Author | Yan Wang1†, Pir Tariq Shah1†, Yue Liu1†, Amina Nawal Bahoussi1, and Li Xing1,2,3* |
| Address | 1Institutes of Biomedical Sciences, Shanxi University, Taiyuan 030006, Shanxi, People’s Republic of China, 2Shanxi Provincial Key Laboratory of Medical Molecular Cell Biology, Shanxi University, Taiyuan 030006, People’s Republic of China, 3Shanxi Provincial Key Laboratory for Prevention and Treatment of Major Infectious Diseases, Taiyuan 030006, People’s Republic of China |
| Bibliography | Journal of Microbiology, 61(9),865-877, 2023, |
| DOI | 10.1007/s12275-023-00078-w |
| Key Words | Echovirus · Phylogenetic analysis · Phylogeographic analysis · Amino acid variation · Genomic recombination |
| Abstract | Echoviruses belong to the genus Enterovirus in the Picornaviridae family, forming a large group of Enterovirus B (EVB) within the Enteroviruses. Previously, Echoviruses were classified based on the coding sequence of VP1. In this study, we performed a reliable phylogenetic classification of 277 sequences isolated from 1992 to 2019 based on the full-length genomes of Echovirus. In this report, phylogenetic, phylogeographic, recombination, and amino acid variability landscape analyses were performed to reveal the evolutional characteristics of Echovirus worldwide. Echoviruses were clustered into nine major clades, e.g., G1–G9. Phylogeographic analysis showed that branches G2–G9 were linked to common strains, while the branch G1 was only linked to G5. In contrast, strains E12, E14, and E16 clustered separately from their G3 and G7 clades respectively, and became a separate branch. In addition, we identified a total of 93 recombination events, where most of the events occurred within the VP1-VP4 coding regions. Analysis of amino acid variation showed high variability in the a positions of VP2, VP1, and VP3. This study updates the phylogenetic and phylogeographic information of Echovirus and indicates that extensive recombination and significant amino acid variation in the capsid proteins drove the emergence of new strains. |