Title Localization of a KEM1::lacZ Fusion Protein in Yeast Cells
Author Kim, Jin Mi * · Gerald R. Fink¹
Address Department of Microbiology, College of Natural Sciences, Chungnam National University; ¹Massachusetts Institute of Technology and Whitehead Institute for Biomedical Research, Cambridge
Bibliography Korean Journal of Microbiology, 32(1),12-19, 1994
Key Words spindle pole body, microtubuled, KEM1::lacZ, intracellular localization
Abstract KEM1 is known to control the spindle pole body or microtubule function, probably in response to the cellular nutritional conditions in Saccharomyces cerevisiae. Transposon insertions were performed in the cloned KEM1 gene using mini-Tn10-LUK element carrying E. coli β-galactosidase structural gene. A collection of ranfom Tn10-LUK insertions defined an approximately 3.5 kb region required for the KEM1 function. From this collection functional KEM1::lacZ protein fusions were identified. Indirect immunofluorescence using anti-β-galacatosidase antibodies localized the KEM1::lacZ fusion protein to the periphery of the nucleus.
Download PDF Kor_320103_12-19.pdf