Title functional characteristics of cytoplasmic and periplasmic photobacterium leiognathi CuZnSOD (PSOD) in Escherichia coli SOD double mutants
Author Kim, Young Gon * · Yang, Mi Kyung
Address Department of biology, College of Natural Sciences, Chosun University
Bibliography Korean Journal of Microbiology, 32(2),139-146, 1994
Key Words superoxide dismutase, subcellular localization
Abstract Protective effects on subcellular localization of Photobacterium leiognathi CuZnSOD(PSOD) were examined in Escherichia coli SOD mutant cells on the treatment of paraquat, heat shock (37℃→42℃), hydrogen peroxide and copper sulfatem respectively. The physiological characteristics of the periplasmic and cytoplasmic PSOD localized differently are dependent on the conditions in this experiment. Cells expressing SOD periplasmically in the treatments of paraquat and H_2O_2 respectively were somewhat better protective effects cells expressiong SOD cytoplasmically at comparable level and SOD expression level showed, the most consistently important variable. However, this was reversed in the treatments of heat shock and CuSO_4, respectively.
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