| Title | Evidence that a Plasmid Encodes Fenes for Metabolism of Malonte in Pseudomonas fluorescens | ||
| Author | Kim, Yu Sam * · Kim, Eun Joo | ||
| Address | Department of Biochemistry, College of Science Bioproducts Research Center, Yonsei University | ||
| Bibliography | Korean Journal of Microbiology, 32(3),192-197, 1994 | ||
| DOI | |||
| Key Words | malonate, plasmid, Pseudomonas fluorescens | ||
| Abstract | Pseudomonas fluorescens which is able to utilize malonate as a sole carbon source was found to contain a novel 60kb plasmid, which encodes the genes for the proteins to assimilate malonate, including malonate decarboxylase and acetyl-CoA synthetase. The evidence is as follow: The Pseudomonas cured with mitomycin C was unable to grow on malonate-medimu as well as it lost plasmid. The plasmid isolated from the pseudomonas could be introduced into E. coli strain JM103 and DH1 by transformation, The transformed E. coli was able to grow on malonate-medium and could transmit its plasmid back to the cured P. fluorescens by conjugation. The existence of the plasmid in the transformed E. coli was confirmed by hybridization with a labeled probe prepared from 12kb segment of the plasmid. Dot hybridization showed that the copy number of the plasmid in the transformed E. coli is at least 13 times higher than in the wild tyoe O. fluorescens. The two key enzymes, malonate decarboxylase and acetyl-CoA synthetase, were inducible by malonate in the transformed E. Coli. | ||
| Download PDF | Kor_320304_192-197p.pdf | ||