| Title | A possible role of lipopolysaccharides in the prevention of lysosome0symbiosome fusion as studied by microinjection of an anti-LPS monoclonal antibody | ||
| Author | Choi, Eui Yul | ||
| Address | Department of Genetic Engineering, Hallym University | ||
| Bibliography | Korean Journal of Microbiology, 32(4),280-284, 1994 | ||
| DOI | |||
| Key Words | Monoclonal antibodies, microinjection, lysosome fusion, lipopoysaccharides, symbiosis | ||
| Abstract | Lack of lysosomal fusion with symbiosome in symbiot0bearing Amoeba proteus may be due either to the presence of a component in the symbiosome membrane or to the absence of a component needed in the fusion process. Using monoclonal antibody as a probe, lipopolysaccharides were identified as symbiosome-membrane components contributed by symbionts and were found to be exposed on the cytoplasmic side of the membrane. In order to test whether lipopolysaccharided antibody was microinjected and processed for double immunostaining in conjunction with anti-lysosome antibody as a lysosome-fusion indicator. Microinjection of the anti-LPS antibody caused symbiosomes to fuse with lysosomes, suggesting that X-bacterial lipopolysaccharided could be ‘fusion-preventing’ factors. | ||
| Download PDF | Kor_320406_280-284p.pdf | ||