Title Biological characteristics of antifungal agents produced by and Actinomyces isolate
Author Jeong, Ga Jin * · Hah, Yung Chil · Kang, Hyen Sam
Address Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University
Bibliography Korean Journal of Microbiology, 32(6),455-463, 1994
Key Words Actinomyces naeslundii G-10, antifungal activity
Abstract Of a large number of Actinomyces spp. Screened from soil for their antifungal activity, a strain referred to as G-10, identified as Actinomyces naeslundii, was found to produce potent antifungal agents against various species of fungi and even some bacteria. Even though it was originally isolated from natural environment, G-10 grew fairly well at 37℃ as well as at 30℃. The maximum antifungal activity, however, was detected from 3~5 day shaking culture at 30℃. The initial pH value of 5.0 was the most optimal for both cell growth and antifungal activity. Different sources of carbon influenced only on cell growth and it is not likely that they have any direct correlationship to antifungal activity. Soybean meal was a good nitrogen source for growth but had no direct stimulatory effect on production of antifungal agent. In general, nutritional requirement does not seem to have any direct effect for the activity. The principal inhibition was exerted via prohibiting the mycelial growth of filamentous fungi and budding in single cell fungi. The concentrated culture supernatant has anti-fungal activity equivalent to 20,000 U/ml nystatin. Pathogenic fungi were relatively more resistant than non-pathogenic ones. Self-inhibition, consequently the population growth inhibition, could be detected when they were densely grow on solid media.
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