Title Isolation and Partial Characterization of a New Escherichia coli Bacteriophage E3
Author Hong, Seung Hee · Cho, Nam Young¹ · Jeong, Ga Jin *
Address Department of Microbiology, and Research Center for Molecular Microbiology, Seoul National University
Bibliography Korean Journal of Microbiology, 32(6),464-470, 1994
Key Words New bacteriophage, E. coli, promoter, plaque
Abstract Bacteriophages were screened from aquatic environment around the campus of Seoul National University. Among several phages isolated, one bacteriophage infecting Escherichia coli produced unusually large plaques upon overnight incubation. Since we planned to screen phages with interests in microbial researches, this phage was reffered to as E3 and characterized morphologically and physiologically. The new bacteriophage E3 has very similar morphology to lambdoid. Other characteristics studied so far clearly showed that it is rather likely to have E3 as a member of Styloviridae(15). From electron microscopic studies, it was found that E3 has icosahedral head of 40~45nm in width and 155~150nm long flexuous tail. E3 has host specificity to E. coli only, and its genome was identified as a double stranded linear DNA with 43kb. SDS-polyacrylamide gel electrophoresis showed that there are more than seven polypeptide bands as structural proteins, and all were actively stained with polyclonal antibodies conjugated with horse radish peroxidase(HRP). The most abyndant protein in detected amount was of 33 Kd m.w. Some restriction endonucleases cut E3 DNA into several fragments(EcoRI, >18; RsaI, >19; HpaII, >21; and HinfI, >21), while many others in common use such as BamHI, HindIII, AvaII, KpnI, PvuII, and SalI have no reactive site at all. Studies on genetic analysis and physical map construction are still under investigation in the hope to find out some possibilities for the application of E3 to microbial genetic researches in identification of new strong promotersm in applacation of bacteriophages for bacteriological control in environmentm or in basic studies on host parasite relationship.
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