Title Virulence evaluation of vibrio anguillarum in accordance with hemolytic activity on human red blood cells
Author Choe, Young Chool · Jeong, Ga Jin *
Address Department of Microbiology, and Research Center for Molecular Microbiology, Seoul National University
Bibliography Korean Journal of Microbiology, 32(6),539-544, 1994
Key Words V. anguillarum, vibriosis, hemolysis, virulence, human RBC
Abstract Vibriosis caused by Vibrio anguillarum has been intensively studied for the understanding of the virulence mechanism. High efficiency iron sequestering system, serum resistance, proteolytic enzyme, and so forth have been reported. It is found that virulent strain of V. anguillarum cleary lysed RBCs from human. The intensity of hemolysis was quite correlated to the degree of virulence. Among various bacterial media, the highest titre of extracellular hemolytic activity of V. anguillarum strain V7(NCMB6; J01) was observed in BHI medium. The production of hemolysin was initially detected when the growth reached stationary phase. Hemolytic activity was detected using RBCs from various animals as well as murine splenocytes. We now report that presence and incidence of virulence could be detected by hemolysis using human RBCs, and this system, as an experimental model, could be applied to studies for understanding pathogenic mechanisms of vibriosis.
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