| Title | Calcium response of CHSE following infection with infectious pancreatic necrosis virus (IPNV) | ||
| Author | Kang, Kyung Hee1 · Park, Kee Soon1 · Yoon, Joo Hyun1 · Lee, Chan Hee1,2 * | ||
| Address | 1Department of Microbiology, College of Natural Science, Chungbuk National Universuty; 2Research Center for Molecular Microbiology, Seoul National University | ||
| Bibliography | Korean Journal of Microbiology, 31(1),79-84, 1993 | ||
| DOI | |||
| Key Words | IPNV, calcium, CHSE cells | ||
| Abstract | Infection of Chinook Salmon Embryo (CHSE) cells with IPNV resulted in a significant decrease in intracellular free calcium concentration ([Ca^2+] i) compared to mock-infected cells. The degree of the decrease in [Ca^2+] i was dependent on the amount of input virus, and treatment of IPNV-infected CHSE cells with metabolic inhibitors such as cyloheximide cordycepin partially reversed the decrease in [Ca^2+] i in IPNV-infected cells. Inactiation of PINV with UV also abolished IPNV-induced decrease in [Ca^2+] i. These data suggest an active role of IPNV in the decrease of [Ca^2+] i in the infected CHSE cells. The importance of the decrease in [Ca^2+] could be supported by the finding that the production of IPNV plaques increased in the cells treated with verapamil, a calcium influex blocker, and by lowering the concentration of extracellular calcium. Decreased production of IPNV plaques was observed by elevating the extracellular calcium. Thus, it is suggested that IPNV induced a decreased in [Ca^2+] i and the decrease in [Ca^2+] i may plan an importat role in efficient replication of IPNV. | ||
| Download PDF | Kor_310112_79-84p.pdf | ||