| Title | Terminal amino acid sequences of alkaline amylase from alkalophilic bacillus sp. MB 809 and their homology | ||
| Author | Bae, Moo * · Kang, Soo Kyung | ||
| Address | Department of Biological Science, Ewha Womans University; Rearch cCenter for Molecular Microbiology, Seoul National University | ||
| Bibliography | Korean Journal of Microbiology, 31(2),175-178, 1993 | ||
| DOI | |||
| Key Words | Alkalophilic Bacillus sp., alkaline amylase, amino acid sequence | ||
| Abstract | Alkaline α-amylase expressed in the transformant, Baciollus subtills MB809, containing alkaline amylase gene cloned from alkalophilic Bacillus sp. AL-8, was purified through for step separation processes. The purified alkaline α -amylase had molecular weight of app[roximately 59,000 daltons on SDS-PAGE and Sephaex G-100 gel filtration. Amino acid sequence of terminal portion of the enzyme was analyzed with pure amylase eluted form the SDS-PAGE gel. N-terminal amino acid sequence of α-amylase was determined by the Edman degradation method and resulted in NH_2-ser-thr-ala-pro-ser-(ile)-lys-ala-gly-thr-(ile)-leu. For C-terminal amino acid sequencing, purified α-amylase was digested with carboxypuptidase A and B, and reverse-phase HPLC gradient elution system resulted in -thr-trp-pro-lys-COOH. | ||
| Download PDF | Kor_310211_175-178p.pdf | ||