| Title | Isolation and Characterization of a Restricted Facultatively Methyloptrophic Bacterium Methylovorus sp. Strain SS1 | ||
| Author | Seo, Sung A. · Kim, Young M. * | ||
| Address | Department of Biology, College of Science, Yonsei University | ||
| Bibliography | Korean Journal of Microbiology, 31(3),179-183, 1993 | ||
| DOI | |||
| Key Words | methanol, methylotroph, restricted facultative methylotroph, Methylovorus sp. strain SS1, ribulose monophophate pathway, extracellular polysaccharide | ||
| Abstract | A restricted facultatively methanol-oxidizing bacterium, Methylovorus sp. strain SS1, was isolate dfrom soil samples from Kuala Lumpur, Malaysia, through methanol-enrichment culture technique. The isolate was nonmotile Gram-negative rod and did not have complex internal membrane system. The colonies were small, pale-yellow, and raised convex with entire margin. The cell did not produce any spores and capsular materials. The cell was obligately aerobic and exhibited catalase, but no oxidase, activity. Plasmid, carotenoid pigment, and poly-β-hydroxybutyric acid were not found. The guanine plus cytosine content of the DNA was 55%. The isolate was found to grow only on methanol methylamine, or glucose. Growth factors were not required. Cells growing on methanol was found to produce extracellular polysaccharides containing glucose, lactose, and fructose. Growth was optimal (t_d= 1.7) with 0.5%(v/v) methanol at 40℃ and pH 6.5. No Growth was observed at over 60℃. Cell-free extracts of the methanol grown cells exhibited the phenazine methosulfate-linked methanol dehydrogenase activity Methanol was found to be assimilate dthrough the ribulose monophosphate pathway. | ||
| Download PDF | Kor_310301_179-183p.pdf | ||