| Title | Inhibitory actions of mycotoxins on brain γ-aminobutyrate transaminase | ||
| Author | Lee, Su-Jin1,2 · Lee, Kil Soo2 · Choi, Soo Young1 * | ||
| Address | 1Department of Genetic Engineering; 2Department of Biology, College of Natural Sciences, Hallym University | ||
| Bibliography | Korean Journal of Microbiology, 31(3),224-229, 1993 | ||
| DOI | |||
| Key Words | GABA transaminase, neurotransmitter, mycotoxin, patulin, penicillic acid. | ||
| Abstract | GABA transminase (4-aminobutyrate aminotransferase), which catalyzes the breakdown of the major inhibitory neurotransmitter, GABA, in mammalian brain, was inactivated by preincubation with the mycotoxin patulin. The time course of the reaction was significantly affected by the substrate .alpha.-ketoglutarate, which aforded complete protection against the loss of catalytic activity. The recovery from the inhibition of patulin by the addition of dithiothreitol (DTT) supports that patulin reacts with the sulfhydryl residue in the catalytic domain of the enzyme. The reconstitution of the reduced enzyme and apoenzyme with pyridoxal-5-P(PLP) was inhibited by another mycotoxin, penicilic acid. This mycotoxin may interact with lysyl residue of the enzyme. Therefore, it is postulated that the critical sulfhydryl and lysyl residues in the catalytic domain of the enzyme react with mycotoxin patulin and penicillic acid, respectively. | ||
| Download PDF | Kor_310308_224-229p.pdf | ||