Title Characteristics of a new obligate methanol-oxidizing bacterium
Author Kim, Si Wouk * · Park, Yong Ha1
Address Department of Environmental Science, Chosun University; 1Bioinformatics and Sysmatic Program, GERI, KIST
Bibliography Korean Journal of Microbiology, 31(4),261-266, 1993
Key Words obligate methylotrophic bacterium, ribulose monophosphate pathway, Methylo-bacillus methanolovorus
Abstract A new methyltrophic bacterium which utilizes methanol as a sole source of carbon and energy was isolated from soil. It was Gram-negative, nonmotile, nonspore-forming rod, and strictly aerobic bacterium. Catalase and oxidase activities were present. Nitrate was reduced to nitrite. Vitamins and other growth factors were not required. Generation time was 1.6 hr under the optimal condition. The isolate assimilated methanol via the ribulose mono-phosphate pathway (Enter-Doudoroff varient) and did not have .alpha.-ketoglutarate dehydrogenase. It assimilated ammonia through glutamate dehydrogenase. The guanine plus cytosine content of the DNA was 61.0 mol%. The celular fatty acid composition was primarily straight-chain saturated C_16:0 acids (palmitic acids) and unsaturated C_16:1 acid (palmitoleic acids), and the isolate also contained two unidentified C_17 branched fatty acids. The major ubiquinone was Q-8, and Q-6 and Q-7 were present as minor components. Phosphatidylethanolamine and phosphatidylglycerol were predominantly present, and diphosphatidyglycerol was also detected. Based on the physiological and biochemical properties, the isolate was assigned to a novel species of the genus Methylobacillus, Methylobacillus methanolovorus sp. nov.
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