| Title | Southern Hybridization Analysis and Identification of the Junction Fragments of Mitochondrial DNA Large-Deletion Mutants in Saccharomyces cerevisiae | ||
| Author | Kim, Sang-Ho | ||
| Address | Department of Biology, Taegu University, Kyungsan-Kun | ||
| Bibliography | Korean Journal of Microbiology, 31(5),370-375, 1993 | ||
| DOI | |||
| Key Words | yeast mtDNA, oxi3 gene, site-specific deletion, D-class deletion, Southern hybridization analysis, junction fragments | ||
| Abstract | In a genetic mapping of random collection of mitochondrial large-deletion mutants(mit-del mutants) of Saccharomyces cerevisiae strain 777-3A, it was found that large-deletions (A, B, C and D class, etc.) were exceptionally frequent in the oxi3 gene, a large mosaic gene coding for subunit I of cytochrome oxidase. The nature of mit-del mutations of four D-class mutants was physically confirmed by Southern hybridization analysis and the junction fragments of the D-class deletions were identified. Two different sizes of the junction fragments were found and, therefore, D-class mutants were different in physical terms, although there was no difference between them in genetic mapping, apparently because there was no mit-marker discriminating these D-class mutants. | ||
| Download PDF | Kor_310503_370-375p.pdf | ||