| Title | Cholera Toxin B Subunit Enhances IgA Isotype Switching in the Presence of Interleukin-2 at the Clonal Level | ||
| Author | Kim, Pyeung-Hyeun * · Yie, Se Won | ||
| Address | Department of Microbiology, College of Natural Sciences, Kangwon National University | ||
| Bibliography | Korean Journal of Microbiology, 31(6),495-503, 1993 | ||
| DOI | |||
| Key Words | cholera toxin, IL-2, TGF-β1, B cell, IgA, isotype switching, limiting dilution | ||
| Abstract | Cholera toxin(CT) has important effects on expression of the IgA isotype and is known to be a strong mucosal adjuvant. The present study asked whether cholera toxin and its subunits can increase the IgA clonal precursor frequency, clonal burst size or both among populations of murine B cells. LPS-activated surface IgA negative (sIgA-) spleen B cells were cultured presence or absence of IL-2, and then assayed for IgA producing cells using an enzyme-linked immunospot(ELISPOT) assay. CTB in the presence of IL-2, significantly increased the precursor frequency of IgA-producing cells. Neither CTB alone nor IL-2 alone altered IgA B cell precursor frequency. In contrast, CTB and IL-2, in combiantion, did not alter the burst size of IgA-producing clones and did not increase the rate of IgA secretion by IgA-producing B cells. Taken together, these data indicate that CTB and IL-2 in combination increase switching of surface IgA-B cells to expression and secretion of the IgA isotype. | ||
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