| Title | A New Bioluminescent Assay of Cholesterol Esterase Using Luminescent Marine Bacterium Vibrio harveyi mutant M-17 | ||
| Author | Cho, Ki Woong | ||
| Address | Marine Biotechnology Research Group, Korea Ocean Research and Development Institute | ||
| Bibliography | Korean Journal of Microbiology, 31(6),532-536, 1993 | ||
| DOI | |||
| Key Words | cholesterl esterase, bioluminescence, Vibrio harveyi mutant M-17 | ||
| Abstract | A new bioluminescent assay method for the activity of cholesterol esterase (cholesteryl ester hydrolase, EC 3.1.1.13) was developed using bioluminescent marine bacteria. A dark mutant of Vibrio harveyi (designated as M-17) was used for this assay. It does not emit high level of light in itself, but in the presence of myristic acid it gives normal level of light as wild type cell. Cholesterol myristate and Bovine pancreas cholesterol esterase were used for this demonstration and the product, myristic acid, was quantitated with V. harveyi mutant M-17. Down to 5nM of myristic acid produced could be detected. It was proved that this method was rapid, reliable, and easy to perform and safe. | ||
| Download PDF | Kor_310611_532-536p.pdf | ||