| Title | Polyamine, Cytochrome c and Enzymes Involved in the Utilization of Methanol in Methylobacterium extorquens AM1 Growing at Different Temperatures | ||
| Author | Park, Ki Jung · Lee, Sun Hee · Kim, Young Min * | ||
| Address | Department of Biology, Yonsei University | ||
| Bibliography | Korean Journal of Microbiology, 31(6),537-543, 1993 | ||
| DOI | |||
| Key Words | Methylobacterium extorquens, methylotroph, methanol, polyamine, temperature, cytochrome c, methanol dehydrogenase, hydroxypyruvate reductase | ||
| Abstract | The generation times of Methylobacterium extorquens AM1 growing on methanol (0.5%, v/v) at pH 7.0 at 18℃ was found to contain more amount of prtrescine that that grown at 30℃ or 36.5℃. Both spermidine and spermine were found to be most abundant in cells grown at 30℃. Cells grown at the three different temperatures exhibited strong methanol dehydrogenase(MDH) activity at the mid-exponential growth phase. The amount of MDH was almost same in cells growing at all growth phases at different temperatures. The c-type cytochromes were most abundant in cells growing at the stationary phae. Cells grown at 18℃ contained less amount of cytochrome c than those grown at other temperatures. The rate of growth of cells growing at 36.5℃ in the medium supplemented with prtrescine, spermidine, or spermine was slightly faster (t_d=20h) than that of cells growing in the absence of the polyamines. Cells grown at 36.5℃ in the presence of exogeneously added spermidine or spermine contained increased amount of spermidine and spermine but contained decreased amount of prtrescine. Putrescine added into the medium was found to increase the level of spermidine and spermine but did not affect that of putrescine. Prtrescine added into the medium did not affect MDH activity but slightly increased hydroxypyruvate redcutase activity. Cells grown in the presence of spermidine or spermine exhibited MDH activity lower than that of the cells grown in the absence of the exogeneously added polyamines. It was found that polyamines added into the medium did not affect the level of MDH but increased that of c-type cytochromes. Spermidine, putrescine, and spermine were found to show no effect on the activity of MDH and hydroxypyruvate reductase in vitro. | ||
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