| Title | Studies on KEM1 gene controlling mitotic cell division in yeast molecular cloning of a high copy suppressor(ROK1) of kem1 | ||
| Author | Kim, Sanghyeon · Kim, Jinmi | ||
| Address | Department of Microbiology, College of Natural Sciences, Chungnam National University | ||
| Bibliography | Korean Journal of Microbiology, 30(1),37-41, 1992 | ||
| DOI | |||
| Key Words | KEM1, cell division cycle, Saccharomyces cerevisiae, microtubules, spindle pole body, high copy suppressor | ||
| Abstract | The KEM1 gene is known to affect microtubule and spindle pole body function during the cell division cycle in Saccharomyces cerevisiae. To identify new genes with functions similar or related to those of KEM1, we isolated a high copy suppressor gene (ROK1) that suppresses the kem1 mutation when cloned on a high copy number plasmid but not on a low copy number plasmid. Two clones which suppress both the benomyl hypersensitivity and the Kar^- enhancing phenotype of kem1 null mutation were isolated and were shown to have a 9.0 kb identical insert by restriction endonuclease analysis. The restriction map constructed indicates that this suppressor gene, ROK1 is not KEM1. Subcloning experiments suggest that the functional region of ROK1 is at least 3.0kb in size. | ||
| Download PDF | Kor_300105_37-41p.pdf | ||