Title Expression and secretion of heterologous protein in yeast
Author Kim, Moo Kyum · Song, Moo Young · Lee, Kee Nyung * · Yu, Myeong Hee * · Kim, Jin Mi
Address Department of Microbiology, College of Natural Sciences, Chungnam National University; * Genetic Engineering Research Institute, KAIST
Bibliography Korean Journal of Microbiology, 30(2),108-112, 1992
Key Words Secretion, Heterologous protein, α1-antitrypsin, Signal sequence, Yeast
Abstract To investigate the expression and the secretion of heterologous proteins in yeast, we constructed an yeast secretion vector and produced a human secretory protein, α1-antitrypsin (α-1-AT), from yeast cells. The secretion vector pGAT8 was constructed by inserting the signal sequence of yeast acid phosphatase gene (PH05) into the α1-AT expression vector pGAT6 which contained α-1-AT cDNA fused to GAL10-CYC1 promotor. The α-1-AT was produced efficiently in the yeast cells transformed with plasmid pGAT8, which was confirmed both by the α-1-AT activity assay and by the immunoblot method using α-1-AT antibody. We also showed the secretion of α-1-AT into the culture media and into the periplasmic space by immunoblot.
Download PDF Kor_300207_108-112p.pdf