Title cDNA cloning and nucleotide sequence determination for VP7 coding RNA segment of human rotavirus isolated in Korea
Author Kim, Young Bong1 · Kim, Kyung Hee2 · Yang, Jai Myung3 *
Address 1Department of Genetic Engineering, Sung Kyun Kwan University; 2Department of Microbiology, School of Medicine, Hanyang University; 3Biology Department, Sogang University *
Bibliography Korean Journal of Microbiology, 30(5),397-402, 1992
Key Words rotavirus, VP7, PCR, cDNA, Sequence homology
Abstract The cDNA of RNA segment coding for VP7 of human rotavirus isolated from patient's stool at Seoul area was synthesized, amplified by polymerase chain reaction, field in with Klenow fragment of DNA polymerase I and cloned into pUC19. The cDNA sequence was determined and compared with that of VP7 coding RNA segments of group A rotaviruses isolates in foreign country. Over 90% sequence homology was found with serotyppe I sepcific WA1 and RE9 strains. Comparative analysis of the deduced amino acid sequences within the two variable regions (amino acid residue 87 through 101 and 208 through 221) with WA1 and RE9 strains also showed high degree of sequence similarity with each other.
Download PDF Kor_300511_397-402p.pdf