| Title | Escherichia coli GroEL was Induced by the Expression fo the Cloned Bacillus megaterium ATCC14945 Penicillin G Acylase Gene | ||
| Author | Kang, Joo Hyun · Kim, Jung Sun · Hahm, Kyung Soo1 · Yoo, Ook Joon * | ||
| Address | Department of Life Science, Korea Adcanced Institute of Science and Technology; 1Genetic Engineering Research Institute, KIST | ||
| Bibliography | Korean Journal of Microbiology, 30(6),421-424, 1992 | ||
| DOI | |||
| Key Words | GroEL, penicillin G acylase, Escherichia coli | ||
| Abstract | Escherichia coli JM83 harboring penicilin G acylase gene of Bacillus megaterium ATCC14945 produced a protein in large amount (>20% of the total protein). The protein was identified as GroEL, one of the E. coli heat shock protein, by N-terminal amino acid sequence analysis. It was found that GroEL was induced by the expressed foreign penicilin G acylase at both 27 and ℃. | ||
| Download PDF | Kor_300601_421-424p0.pdf | ||