Title |
Effects of gene expression of photobacterium leiognathi CuZn superoside dismutase (PSOD) by lacZ promotor control under oxidative stress |
Author |
Young Gon Kim |
Address |
Biology, Natural Sceinces, Chosun University |
Bibliography |
Korean Journal of Microbiology, 30(6),460-465, 1992 |
DOI |
|
Key Words |
superoxide dismutase, oxygen radical, gene expression, lac promoter |
Abstract |
The effect of PSOD expression on lacZ-sodP fusion (pYK4) was explored in Escherichia coli sodA sodB mutants (QC774) under oxidative stress. In this system, although β-galactosidase activity was not fully induced by isopropyl-1-thio-β-galactosidase (IPTG) and was inhibited by glucose, functional PSOD was under lacZ promotor control and was induced by IPTC, lactose, PQ and copper isons, finally, the results show that higher PSOD expression level was consistently important in defending against superoxide radicals. |
Download PDF |
Kor_300608_460-465p.pdf |