| Title | Isolation and regeneration of protoplast in streptomyces antibioticus | ||
| Author | Yeo, Myeonggu · Koh, Hancheol · Park, Kyoungsu1 · Park, Yeal | ||
| Address | Department of Genetic Engineering; 1Department of Biology, College of Natyral Sciences, Chosun University | ||
| Bibliography | Korean Journal of Microbiology, 30(6),514-518, 1992 | ||
| DOI | |||
| Key Words | Streptomyces antibioticus, protoplast, oleandomycin | ||
| Abstract | The present study has been perromed to investigate the optimal conditions for protoplast formation and regeneration of oleandomycin-producing Streptomyces antibioticus (S. antibioticus) KCTC 1081. Mycelia were grown in YME medium containing 0.2% (w/v) glycine and converted into the protoplast by incubating at 35℃ for 60 minutes in protoplast buffer (P buffer) containing 4 mg/ml lysozyme. The reversion of protoplasts to the normal filamentous state was examined by the growth on various synthetic agar media. A high reversion rate was obtained by incubating the protoplasts on a hypertonic agar medium containing 20 mM Mg^++, 5 mM Ca^++ and 0.3 M sucrose at 28℃ for 5 days. From these experiments, we established the improved regeneration medium and a protocol which supports higher and more consistent levels of regeneration of S. antibioticus protoplasts. The regenerant showed an increased antimicrobial activity compared with that of the initial strain. | ||
| Download PDF | Kor_300617_514-518p.pdf | ||