| Title | Purification and properties of glucoamylase form yeast candida tsukuaensis | ||
| Author | Kim, Sanga Moon * · Bai, Suk · Chung, Hee Young · Park, Jong Chun · Lee, Jin Jong · Kim, Dong Ho · Song, Myung Hee · Chun, Soon Bai | ||
| Address | * Department of Spectacles and Opticd, Kwang-ju Health Junior College; Department of Microbiology, College of Natural Sciences, Chonnam National University | ||
| Bibliography | Korean Journal of Microbiology, 30(6),519-523, 1992 | ||
| DOI | |||
| Key Words | Candida tsukubaensis, glucoamylase | ||
| Abstract | The glucoamylase of Candida tsuubaensis was purified to homogeneity form culture filtrate by means of ultrafiltration, Sephacryl S-200 gel filtration and Sp-Sephadex C-50 chromatography. The purified enzyme was a glycoprotein with a molecular mass of approximately 50 kDa, which was a monomeric protein. Km values were 5.8 mg/ml for soluble starch and 0.04 mM for maltose. Glucoamylase also released only glucose from both pullulan and isomaltose. The analysis of amino acid composition revealed that the enzyme contained a high content of acidic and polar amino acids. In addition, Western blotting analysis indicates that C. tsukubaensis glucoamylase is resistant to glucose repression. | ||
| Download PDF | Kor_300618_519-523p.pdf | ||