| Title | 대장균에서 사람의 면역결핍 바이러스-1 epitopes 클로닝과 발현에 대한 연구 Cloning and expression of human immunodeficiency virus-1 epitopes in escherichia coli | ||
| Author | 유향숙 · 장원희 · 박희동 · 현상원 · 남상욱 · 이영익 * | ||
| Address | 한국과학기술연구원; 유전공학연구소; 분자유전학실 | ||
| Bibliography | Korean Journal of Microbiology, 29(1),1-7, 1991 | ||
| DOI | |||
| Key Words | HIV-1 epitopes/ tac and trp expression vector/ immunodominant epitopes/ hydrophobic region | ||
| Abstract | Human immunodeficiency virus type 1 (HIV-1) causes a deadly infectious disease, Acquired Immunodeficiency Syndrome (ADIS). As a first step to develop a reliable and fast diagnostic procedure for HIV-1 infection, we cloned various immunodominant epitopes of HIV-1 in bacterial expression vectors containing tac or trp promoter. While the protein level of direct expression of gp160 was low, trp E fused gp120, gp41 and p17-p24 were produced at high levels (15-30% of total bacterial proteins) in E. coli. Since gp120 and gp41 contain relatively conserved regions which can react with antibodies in the plasma from most of HIV-1 infected individuals, these expression clones were used for large preparations of HIV-1 antigens. | ||
| Download PDF | Kor_290101_1-7p.pdf | ||