| Title | Human cytomegalovirus 감염에 대한 파파베린과 뉴클레오사이드 유사체의 항바이러스 효과 Antiviral activity of papaverine and nucleoside analogs on the human cytomegalovirus infection | ||
| Author | 이찬희1 · T. Albrecht2 | ||
| Address | 1충북대학교 자연과학대학 미생물학과; 2Department of Microbiology, University of Texas Branch | ||
| Bibliography | Korean Journal of Microbiology, 29(1),25-33, 1991 | ||
| DOI | |||
| Key Words | HCMV, papaverine, DHPG, acyclovir | ||
| Abstract | Antiviral activities of papaverine and nucleoside analogs, 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (DHPG) and acyclovir, against human cytomegalovirus (HCMV) infection were compared in vitro. Papaverine and DHPG were effective in reducing infectious HCMV yields with ED_50S (effective dose 50: the concentraion at which 50% of virus yields was obtained) of approximately 1.02 and 0.45uM, respectively; while acyclovir was less effective with an ED_50 of about 10.4 uM. The relative cytotoxicity of these drugs was evaluated under the same conditions used to measure infectious HCMV yields. Papaverine and DHPG demonstrated little cellular toxicity as measured by their effect on the viability of confluent cells at concentrations in the range of those demonstrating potent inhibition of HCMV replication. Similarly, protein synthesis was largely unaffected by these drugs in stationary mock-infected cells as measured by the incorporation of isotopically labelled amino acids. In contrast, cellular DNA synthesis was invariably reduced in the presence of either drug. HCMV-specific DNA synthesis was also strongly inhibited by papaverine and DHPG. | ||
| Download PDF | Kor_290104_25-33p0.pdf | ||