| Title | CDDP를 처리한 bluetongue virus core의 전사저해 cis-Diamminedichloroplatinum (II) (CDDP) inhibits bluetongue virus (BTV) core associated transcriptase activity | ||
| Author | 양재명 · JaRue S. Manning * | ||
| Address | 성균관대학교 유전공학과; * 캘리포니아대학교, 대이비스 | ||
| Bibliography | Korean Journal of Microbiology, 29(1),34-39, 1991 | ||
| DOI | |||
| Key Words | CDDP, Bluetongue Virus(BTV), Transcriptase, Northern blot | ||
| Abstract | The BTV core associated transcriptase activity, assayed by acid precipitable counts, was reduced to an undetectable level after treat the core with 100 uM CDDP. When the RNA transcripts prepared from the CDDP treated BTV core were analysed on agaroseurea gel, it was observed that the band intensity of the large size RNA was reduced while the band intensity of the small size RNA was enhanced. Northern blot analysis showed that much of the small size RNAs appeared to be prematurely terminated transcripts. These results suggest that CDDP adduction to the template RNA blocks chain elongation process of the virion bound transcriptase that is ultimately responsible for the inactivation of BTV infectivity. | ||
| Download PDF | Kor_290105_34-39p.pdf | ||