| Title | 하천에서 분리한 비소 내성세균의 유전적 특성 Genetic characteristics of arsenic compounds-resistant bacteria isolated from stream water | ||
| Author | 정미경 · 이호자 | ||
| Address | 경희대학교 문리과학대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 29(1),63-68, 1991 | ||
| DOI | |||
| Key Words | Klebsiella pneumoniae, arsenite-resistant plasmid, conjugation, plasmid curing test, heavy metal ions and antibioticd resistance | ||
| Abstract | Several arsenic compound-resistant bacteria were isolated from Jung-Rang stream. The isolates, D-3, D-12, and D-14 were characterized phenotypically and genetically, and identified as Serratia liquefaciens, Klebsiella oxytoca, and Klebsiella pneumoniae, respectively. A plasmid of 67kb was found in Klebsiella oxytoca D-12 and designated as pMH12. Transfer of this plasmid from D-12 to E. coli HB101 was occurred, and the resulting transconjugant strains expressed the same level of heavy metal resistance as the donor strain. The physical presence of this plasmid in transconjugant was detected with agarose gel electrophoresis. Arsenite-sensitive derivatives of isolate D-12 were obtained with Mitomycin C treatment which cured pMH12. Antibiotics and heavy metal resistances were also examined to be used as a proper marker for the isolates in gene cloning. Isolate D-12 has resistance to several heavy metal ions such as Cd^2+, Zn^2+ and Hg^2+. Also, all the other arsenite resistant isolates showed resistance to several heavy metal ions and antibiotics. | ||
| Download PDF | Kor_290109_63-68p.pdf | ||