Title ski^- 기주 세포에서 L-A dsRNA 바이러스의 defective interfering particle을 유도하는 효모 유전자
Author 이현숙
Address 경상대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 29(2),75-79, 1991
DOI
Key Words Yeast killer, Defective interfering particles of L-A, ski^-mutation
Abstract The yeast L-A virus (4.6 kb dsRNA genome) encodes the major coat protein and a "gag-pol" fusion minor coat protein that separately encapsidate itself and M_1, a 1.8 kb dsRNA satellite virus encoding a secreted protein toxin (the killer toxin). The teast chromosomal SKI genes prevent viral cytopathology by lowering the virus copy number. Thus, ski^- mutants are ts and cs for growth. We transformed a ski2-2 virus-infested mutant with a yeast bank in a high copy cloning vector and selected the rare healthy transformants for analysis. One type of transformant segregated M-O L-A-O cells with high frequency. Elimination of the DNA clone from the ski2-2 strain eliminated this phinotype and introduction of the DNA clone recovered from such transformants into the parent ski2-2 strain, or into ski3 or ski6 mutants gave the same phenotype. This killer-curing phenotype was due to the curing of the helper L-A dsRNA virus. The 6.5 kb insert only had this activity when carried on a high copy vector and in ski^- cells (not in SKI^+ cells). This 6.5 kb insert acts as a mutagen on L-A dsRNA producing a high rate of deletion mutations.
Download PDF Kor_290201_75-79p.pdf