| Title | ski^- 기주 세포에서 L-A dsRNA 바이러스의 defective interfering particle을 유도하는 효모 유전자 | ||
| Author | 이현숙 | ||
| Address | 경상대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 29(2),75-79, 1991 | ||
| DOI | |||
| Key Words | Yeast killer, Defective interfering particles of L-A, ski^-mutation | ||
| Abstract | The yeast L-A virus (4.6 kb dsRNA genome) encodes the major coat protein and a "gag-pol" fusion minor coat protein that separately encapsidate itself and M_1, a 1.8 kb dsRNA satellite virus encoding a secreted protein toxin (the killer toxin). The teast chromosomal SKI genes prevent viral cytopathology by lowering the virus copy number. Thus, ski^- mutants are ts and cs for growth. We transformed a ski2-2 virus-infested mutant with a yeast bank in a high copy cloning vector and selected the rare healthy transformants for analysis. One type of transformant segregated M-O L-A-O cells with high frequency. Elimination of the DNA clone from the ski2-2 strain eliminated this phinotype and introduction of the DNA clone recovered from such transformants into the parent ski2-2 strain, or into ski3 or ski6 mutants gave the same phenotype. This killer-curing phenotype was due to the curing of the helper L-A dsRNA virus. The 6.5 kb insert only had this activity when carried on a high copy vector and in ski^- cells (not in SKI^+ cells). This 6.5 kb insert acts as a mutagen on L-A dsRNA producing a high rate of deletion mutations. | ||
| Download PDF | Kor_290201_75-79p.pdf | ||