| Title | 원형질체 융합에 의한 인산염 제거균주의 개량 | ||
| Author | 윤성녀 · 조경주 * 조홍범 · 최영길 | ||
| Address | 한양대학교 자연과학대학 생물학과; *생물교육과 | ||
| Bibliography | Korean Journal of Microbiology, 29(2),117-122, 1991 | ||
| DOI | |||
| Key Words | Aeromonas hydrophila, Acinetobacter calcoaceticus, Escherichia coli HR262/pCE27, phosphate uptake midium(PUM), phosphate releasing medium(PRM), pit, pst. | ||
| Abstract | In order to improve the removal ability of phosphate, Spheroplast fusions were performed among auxotrophic mutants of Aeromonas hydrophila isolated from waste water, named A13 and A14, Aci37 auxotrophic mutant of Acinetobactercalcoaceticus, and auxotrophic E. coli HR262/pCE27 carring pit gene. Eight fusants obtained from this experiment showed different biochemical characteristics. When the rate of phosphate uptake among fusants (F1-F8) was investigated in Phosphate Uptake Medium (PUM), F8 strain showed the highest rate for phosphate removal, 7 times as much as control after two hours incubation. The role of cations (Mg^++, Ca^++, K^+) in phosphate uptade by F8 was also investigated in PUM without each salt. K^+ seemed to be crucial. Being compared with phosphate untake rate in PUM, that in PUM without K^+ was reduced 1.5 times. Therefore, by applying F8 strain and K^+ in practical environmental system, the increased efficiency in phosphate removal can be derived. | ||
| Download PDF | Kor_290208_117-122p.pdf | ||