| Title | Fusarium poae와 fusarium sporotrichioides간의 원형질체 융합 | ||
| Author | 하경란 · 장성렬 * · 민병례 | ||
| Address | 상명여자대학교 생물학과; *한양대학병원 임상교수연구실 | ||
| Bibliography | Korean Journal of Microbiology, 29(2),123-129, 1991 | ||
| DOI | |||
| Key Words | Fusarium poae, Pusarium sporotrichioides, interspecific fusion | ||
| Abstract | In order to develop the protoplast fusion method of the strains of Fusarium, the interspecific protoplast fusion was attempted between Fusarium poae and F. sporotrichioides. Various auxotrophic mutants were isolated by the treatment of N-Methyl-N'-Nitro-N-Nitrosoguanidine. The optimal conditions for the formation and regeneration of protoplasts were examined and the characteristics of a fusant were studied. As a results, protoplasts were readily obtained from 18 hours cultured mycelia by the treatment of driselase for 3 hours and 0.6 M KCl as a best osmotic stabilizer at pH 6.0 for the formation of protoplast. Sucrose was the most suitable for the regeneration. Polyetylene glycol (M.W. 8,000) in CaCl_2-glycine solution was used to induce the protoplast fusion. The interspecific fusion frequency between protoplasts among the auxotrophic mutants of the two strains ranged from 2.7×10^-2 to 5.7×10^-3. DNA content and cellulase activity were rather increased in the interspecific fusant. The lag phase of growth curve was slightly elongated in the fusant. | ||
| Download PDF | Kor_290209_123-129p.pdf | ||