| Title | 콩(glycine max)의 공생균주 bradyrhizobium japonicum SNU001의 특성 | ||
| Author | 고세리 · 박용근 * · 안정선 | ||
| Address | 서울대학교 자연과학대학 생물학과; * 고려대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 29(2),143-147, 1991 | ||
| DOI | |||
| Key Words | Bradyrhizobium japonicum SNU001, Root nodulem Glycin max, Symbiotic genes | ||
| Abstract | The root nodules and Glycine max were classified as determinate nodule based on their morphological characteristics, and isolated endosymbiont as a Bradyrhizobium based on its growth rate and single subpolar flagellum. The isolate was similar to B. japonicum USDA110 in utilization of carbon source, growth at 38℃ and 2% NaCl, production of H_2S and especially in the restriction endonuclease digestion pattern of symbiotic genes, allowing them to be placed in sTI group together. The former, however, grew better than the later in broad pH range from 5.0 to 9.5. Infectivity and effectivity of the isolate were confirmed by inoculation of soybean seedlings with the isolates. Characteristics of the reisolated endosymbiont from induced root nodules were identical to those of the first isolate. From these results, it was confirmed that Bradyrhizobium strain isolated from the root nodules of Glycine max was a real symbiont, and was named B. japonicum SNU001. | ||
| Download PDF | Kor_290212_143-147p.pdf | ||