Title Streptomyces tubercidicus에서 adenine과 histidine에 의한 tubercidin 생합성 조절
Author 유진철 · 하영칠 *
Address 조선대학교 약학대학 약학과; * 서울대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 29(3),160-166, 1991
Key Words S. tubercidicus, tubercidin biosynthesis, regulation, primary metabolites, adenine, histidine
Abstract The regulatory mechanism of tubercidin biosynthesis in Streptomyces tubercidicus was studied. In a wild type strain, addition of adenine and histidine into the medium decreased the tubercidin production by 60-65% and 40%, respectively. The effects of adenine and histidine were alleviated by the addition of inosine monophosphate and 5-aminoimidazole-4-carboxamide ribotide. The production of tubercidin in S. tubercidicus K115 strain (ade^-) was nearly shut off by histidine. In contrast with K115 strain, adenine inhibited the tubercidin biosynthesis in S. tubercidicus K412 strain (his^-). In S. tubercidicus F667 strain (ade^-, his^-), tubercidin production was increased by adenine and histidine. From the effects of adenine and histidine on tubercidin biosynthesis in S. tubercidicus wild type and mutant strains, it became known that feedback control by adenine and histidine of biosynthetic pathwat for purine ribonucleotide and histidine are involved in the regulation of tubercidin biosynthesis.
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