| Title | Dihydrodipicolinate synthetase를 코딩하는 corynebacterium glutamicum의 dapA 유전자의 클로닝 및 발현 | ||
| Author | 오종원 · 한종권 · 이현환 * · 현형환 ** · 이재홍 · 스테판 정1 | ||
| Address | 제일제당(주) 종합연구소; 1유진텍 인터내셔날 미국 | ||
| Bibliography | Korean Journal of Microbiology, 29(4),203-208, 1991 | ||
| DOI | |||
| Key Words | dapA gene, Corynebacterium glutamicum, Cloning, Expression | ||
| Abstract | The dapA-complementing gene (L-2, 3-dihydrodipicolinate synthetase: DHDP synthetase, dapA) has been cloned by using a cosmid genomic bank of Corynebacterium glutamicum JS231 that is a lysine overproducer, AEC (s-(2-aminoethyl)-L-cysteine) resistant mutant. By enzymatic deletion analysis, the DNA region complementing the escherichia coli dapA host could be confined to 4.5kb SalI-generated DNA fragment. This DNA fragment was inserted into the C. glutamicum/E. coli shuttle vector pECCG117 to construct pDHDP5812. The specific activity of DHDP synthetase detected in C. glutamicum JS231/pDHDP5812 was increased about 10 fold above that of C. glutamicum JS231. The addition of leucine during growth did not repress the expressin of dapA, and the enzyme activity was not inhibited by lysine. | ||
| Download PDF | Kor_290401_203-208p.pdf | ||